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Image Search Results
Journal: Journal of invertebrate pathology
Article Title: First description of French V. tubiashii strains pathogenic to mollusk: II. Characterization of properties of the proteolytic fraction of extracellular products.
doi: 10.1016/j.jip.2014.09.006
Figure Lengend Snippet: Fig. 1. (A) SDS–PAGE and (B) azocasein-gel zymography analyses of the ECPs of V. tubiashii 07/118 T2 SDS–PAGE (20 lg of protein/well) was carried out on a 10% polyacrylamide gel. The gel was stained with Sypro Ruby. A zymography gel was carried out in 10% SDS–PAGE co-polymerized with 5 mg/ml of azocasein for the detection of protease activity (20 lg of proteins/well); the gel was stained with Coomassie brilliant blue. (C) Digestion profiles of ECPs (5 lg) incubated with oyster collagen (20 lg). The gel was visualized by Coomassie blue. M: Molecular weight markers. Arrows showed the molecular weight of the most detected proteins.
Article Snippet: Twenty micrograms of crude ECPs was analyzed on a 10% SDS–PAGE gel according to the method of Laemmli (1970) and visualized by
Techniques: SDS Page, Zymography, Staining, Activity Assay, Incubation, Molecular Weight